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Part:BBa_J23069

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-14)


TraGr KO

J23068 Datsenko Wanner on pKD46/Rlambda

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 348
    Illegal suffix found in sequence at 1348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 348
    Illegal SpeI site found at 1349
    Illegal PstI site found at 1363
    Illegal NotI site found at 354
    Illegal NotI site found at 1356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 348
    Illegal suffix found in sequence at 1349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 348
    Illegal XbaI site found at 363
    Illegal SpeI site found at 1349
    Illegal PstI site found at 1363
    Illegal NgoMIV site found at 281
    Illegal NgoMIV site found at 1410
    Illegal NgoMIV site found at 1616
    Illegal NgoMIV site found at 1634
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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