Intermediate

Part:BBa_J23068

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-14)


Intermediate #2 in construction of TraGr cassette

TraG region (J23068) cloned into pSB1A2-Bca9007

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 367
    Illegal suffix found in sequence at 1367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 367
    Illegal SpeI site found at 1368
    Illegal PstI site found at 1382
    Illegal NotI site found at 373
    Illegal NotI site found at 1375
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 367
    Illegal suffix found in sequence at 1368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 367
    Illegal XbaI site found at 382
    Illegal SpeI site found at 1368
    Illegal PstI site found at 1382
    Illegal NgoMIV site found at 300
    Illegal NgoMIV site found at 1429
    Illegal NgoMIV site found at 1635
    Illegal NgoMIV site found at 1653
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1807
    Illegal BsaI site found at 1985
    Illegal BsaI site found at 3053
    Illegal SapI.rc site found at 4135


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