Composite

Part:BBa_J23050

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-10)


[lock3e][RFP]

lock3e-RFP. Plasmid is in a pSB3C6 variant analogous to J23006. There is a Ptet promoter upstream of XbaI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 49
    Illegal EcoRI site found at 2106
    Illegal XbaI site found at 2340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2106
    Illegal SpeI site found at 50
    Illegal PstI site found at 64
    Illegal NotI site found at 57
    Illegal NotI site found at 2112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2106
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 50
    Illegal EcoRI site found at 2106
    Illegal XbaI site found at 2340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2106
    Illegal XbaI site found at 2340
    Illegal SpeI site found at 50
    Illegal PstI site found at 64
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1145


[edit]
Categories
Parameters
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