Composite

Part:BBa_J23047

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-10)


[lock3b][RFP]

lock3b-RFP. Plasmid is in a pSB3C6 variant analogous to J23006. There is a Ptet promoter upstream of XbaI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 50
    Illegal EcoRI site found at 2107
    Illegal XbaI site found at 2341
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2107
    Illegal SpeI site found at 51
    Illegal PstI site found at 65
    Illegal NotI site found at 58
    Illegal NotI site found at 2113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2107
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 51
    Illegal EcoRI site found at 2107
    Illegal XbaI site found at 2341
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2107
    Illegal XbaI site found at 2341
    Illegal SpeI site found at 51
    Illegal PstI site found at 65
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1146


[edit]
Categories
Parameters
None