Plasmid_Backbone

Part:BBa_J23006

Designed by: John Anderson   Group: iGEM2006_Berkeley   (2006-07-01)

Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI

Berkley2006-pJ23006Map.GIF

pSB1A2-derived plasmid for constructing basic biobrick parts under the control of an external promoter. Insertion of parts between the XbaI and SpeI (or) PstI sites places the part under the control of the part BBa_R0040 (Tet) promoter. The resulting plasmid retains the normal sequence context of a R0040.newpart composite with one point mutation changing the ACTAGA linker to TCTAGA.


  • RS 16:09, 12 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is this part (BBa_J23006) and the part is BBa_J23025. The physical DNA associated with this part has sequence BBa_J23006-J23025.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1175
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//plasmidbackbone/libraryscreening/rbscodingsequence
Parameters
chassis
copies
mcsBioBrick friendly
originColE1
resistanceA