Plasmid

Part:BBa_J23001

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-06-20)

pSB3C6-J01022 p15A+CmR plasmid with OnRFP part

Berk2006iGEM-pSB3C6map.gif
pSB3C6 is derived from pACYC184 and contains the p15A origin of replication and a gene for chloramphenicol resistance. The standard Biobrick restrictions sites are unique and present.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1083
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1945
    Illegal SpeI site found at 1084
    Illegal PstI site found at 1098
    Illegal NotI site found at 7
    Illegal NotI site found at 1091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1084
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1084
    Illegal PstI site found at 1098
    Illegal AgeI site found at 794
    Illegal AgeI site found at 906
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1859
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None