Plasmid_Backbone

Part:BBa_J18964

Designed by: Sean Sleight   Group: Affiliates   (2013-06-18)


pGA3K4

Vector with pGA Prefix/Suffix, confers kanamycin resistance, p15a origin of replication, constitutively expresses luxR and araC.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
    Illegal NheI site found at 1384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
    Illegal BglII site found at 4726
    Illegal BamHI site found at 1
    Illegal XhoI site found at 16
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
    Illegal AgeI site found at 1470
    Illegal AgeI site found at 1793
    Illegal AgeI site found at 4610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2498
    Illegal SapI site found at 4592


[edit]
Categories
Parameters
None