Protein_Domain
ZipR34

Part:BBa_J18924

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)

R34 heterodimerizing leucine zipper

Engineered Leu zipper based on VBP B-ZIP domain that forms medium-weak parallel heterodimers with variants E34(X). This zipper corresponds to RR34(A) in Acharya et al and R34 in Bashor et al. Bashor et al. used the three different pairs to tune the affinity of their MAPK feedback device.

Interaction with E34(X) variants:

  • E34(I): Kd = 6.1 nM = J18923
  • E34(V): Kd = 41 nM
  • E34(N): Kd = 810 nM

(CRG-internal ID: rg0112)

References

  • Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375
  • Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/binding
Parameters
None