Composite
RFP-miRtsB

Part:BBa_J176136

Designed by: Karmella Haynes, Daniel Flicker   Group: Haynes Lab   (2011-12-10)

HPK-RFP-miRts BCL2

Red fluorescent protein designed to be repressed by microRNAs (miR's) from luciferase (artificial) and miR-34 (natural)
Alias: DF4



Usage and Biology

Double mCherry red fluorescent protein that enters the nucleus (via the NLS tag) and binds to Gal4 DNA sequences (via Gal4DB). It is designed to be repressed by either miR-34 (via miRts BCL2) or miR-luc (via miRts luc).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 683


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Categories
Parameters
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