Part:BBa_J176125

NLS-pcDNA3.1+

NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 967
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 2316
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal NheI site found at 895
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 2316
  Illegal NotI site found at 958
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal BglII site found at 12
  Illegal BamHI site found at 929
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 967
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 2316
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 967
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 2316
  Illegal NgoMIV site found at 1426
  Illegal NgoMIV site found at 2767
  Illegal NgoMIV site found at 3050
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 882
  Illegal BsaI.rc site found at 4575
  Illegal SapI site found at 3495
  Illegal SapI.rc site found at 2616
  Illegal SapI.rc site found at 2826