Plasmid_Backbone

Part:BBa_J176123

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-10-22)


pcDNA3.1+ hygro

pcDNA3.1+ hygro/ΔpCMV, based on pcDNA3.1+; no promoter; amp, hygro

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal EcoRI site found at 1686
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1776
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal EcoRI site found at 1686
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1776
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal EcoRI site found at 1686
    Illegal BglII site found at 12
    Illegal XhoI site found at 299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal EcoRI site found at 1686
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1776
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal EcoRI site found at 1686
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1776
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 2571
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4096
    Illegal SapI site found at 3016


[edit]
Categories
Parameters
n/apcDNA3.1+ hygro