Plasmid_Backbone
Part:BBa_J176123
Designed by: Brady Laughlin Group: Haynes Lab (2011-10-22)
pcDNA3.1+ hygro
pcDNA3.1+ hygro/ΔpCMV, based on pcDNA3.1+; no promoter; amp, hygro
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal EcoRI site found at 1686
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal PstI site found at 1776 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal EcoRI site found at 1686
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal PstI site found at 1776
Illegal NotI site found at 292 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal EcoRI site found at 1686
Illegal BglII site found at 12
Illegal XhoI site found at 299 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal EcoRI site found at 1686
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal PstI site found at 1776 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal EcoRI site found at 1686
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal PstI site found at 1776
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 2571 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 4096
Illegal SapI site found at 3016
[edit]
Categories
Parameters
n/a | pcDNA3.1+ hygro |