Part:BBa_J176122

pcDNA3.1+ puro

pcDNA3.1+ puro/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, puro
Alias: MV2

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal XbaI site found at 305
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  Illegal NotI site found at 292
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal BglII site found at 12
  Illegal XhoI site found at 299
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal XbaI site found at 305
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal XbaI site found at 305
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  Illegal NgoMIV site found at 737
  Illegal NgoMIV site found at 2148
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1873
  Illegal BsaI.rc site found at 3673
  Illegal SapI site found at 2593