Part:BBa_J176121

V0200

FRT-marked mammalian transfection vector (from Invitrogen pcDNA5 FRT/TO); no promoter; amp, hygro

Usage and Biology

• Used to create a stable insert into a mammalian chromosome
• Must be co-transfected with a plasmid expressing Flp recombinase
• Host cells must carry a FRT target site

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1238
  Illegal XbaI site found at 970
  Illegal SpeI site found at 329
  Illegal PstI site found at 1328
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1238
  Illegal SpeI site found at 329
  Illegal PstI site found at 1328
  Illegal NotI site found at 309
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1238
  Illegal BglII site found at 47
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1238
  Illegal XbaI site found at 970
  Illegal SpeI site found at 329
  Illegal PstI site found at 1328
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1238
  Illegal XbaI site found at 970
  Illegal SpeI site found at 329
  Illegal PstI site found at 1328
  Illegal NgoMIV site found at 831
  Illegal NgoMIV site found at 2093
  Illegal AgeI site found at 396
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 378
  Illegal BsaI.rc site found at 3621
  Illegal SapI site found at 2538