Protein_Domain
mCh

Part:BBa_J176005

Designed by: Karmella Haynes   Group: Haynes_Lab   (2011-09-29)

mCh

Codon optimized mCherry red fluorescent protein


Usage and Biology

The mushroom coral Discosoma sp. is the source of monomeric RFP.

mCherry is a mutant derivative of mRFP. PDB 2H5Q is shown here. N = amino terminus; C = carboxyl terminus.
  • Protein Data Bank ID 2H5Q


"Monomeric cherry" (mCherry) red fluorescent protein is a derivative of monomeric RFP from the mushroom coral Discosoma sp. (Shaner et al. 2004, Shu et al. 2006). This BioBrick was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).

REFERENCES

  1. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 22:1567-1572
  2. Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
  3. Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.















Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//classic/reporter
//function/reporter/fluorescence
Parameters
chassismammalian cells
emission610 nm
excitation587 nm