Regulatory
Ubc

Part:BBa_J176003

Designed by: Karmella Haynes   Group: Haynes_Lab   (2011-09-29)

Ubc

Ubc constitutive promoter


Usage and Biology

Comparison of human promoters in U2OS cells
  • "Weak" expression compared to CMV
  • Constitutive (does not require induction)

The Ubiquitin C (Ubc) promoter drives expression at a level below that of the human phosphoglycerate kinase (HPK) promoter in mammalian cells. Expression of red fluorescent protein and cyan fluorescent protein are difficult to detect via microscopy, but can be measured at levels above background (non-fluorescent cells) via flow cytometry with some adjustment of instrument sensitivity.

REFERENCES

  • Will be added soon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
chassismammalian cells