Composite
Part:BBa_J15510
Designed by: Kwabena O. Duedu, Chris French, Sahreena Lakhundi Group: French Lab (2013-11-25)
Pspac+RBS+cex+RBS+cenA
Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli spac promoter (BBa_J15506). Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 818
Illegal NotI site found at 1799
Illegal NotI site found at 2943 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2856
Illegal BamHI site found at 1992
Illegal XhoI site found at 2354
Illegal XhoI site found at 2603 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 451
Illegal NgoMIV site found at 824
Illegal NgoMIV site found at 1326
Illegal NgoMIV site found at 2134
Illegal NgoMIV site found at 3059 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2038
Illegal BsaI.rc site found at 871
Illegal SapI.rc site found at 954
[edit]
Categories
Parameters
//function/biofuels
//function/degradation/cellulose
//function/degradation/cellulose
None |