Composite

Part:BBa_J15202

Designed by: Chris French   Group: French Lab   (2007-07-13)


rbs+lacZ'

Escherichia coli lacZ' gene encoding the first 77 amino acids of LacZ (beta galactosidase), with a strong synthetic ribosome binding site. Complements the common lacZ-delta-M15 mutation found in many laboratory strains of E. coli, allowing blue-white selection. Note the presence of a SacI site at the 5' end, overlapping the XbaI site; this is preserved when this biobrick is added to any other biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None