Composite

Part:BBa_J15201

Designed by: Chris French   Group: French Lab   (2007-07-13)


rbs+arsR, E. coli chromosomal arsR repressor coding sequence with rbs.

This is the Escherichia coli chromosomail arsR coding sequence encoding the ArsR repressor, which represses the ars promoter in the absence of arsnate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056) attached to a strong synthetic ribosome binding site BBa_J15001. Note the presence of a SacI site before the rbs, overlapping the XbaI site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None