Temporary

Part:BBa_J133650

Designed by: Norman Wang   Group: Presting Lab   (2011-02-17)


Chimerabrick Replacement Cassette

Synthesized oligo intended to fix the original Screening Plasmid 3.0 design

from >MA reporterA NI< reporterB

to reporterA >MA NI< reporterB

The oligos encode Chimerabrick Replacement Cassette insert (cut X N): X rE sP1 > M A N

Replaces X to N region in current construct (cut X N): E X > M A B0032 GFP rE sP1 N I < sP2 rE Hr S P

to: E X rE sP1 > M A N I < sP2 rE Hr S P

Legend:

> = BsmBI

M = MfeI

A = AvrII

N = NheI

I = NsiI

> = BsaI

rE = rnaseE site

sP1 = spacer 1

sp2 = spacer 2

rE = rnaseE site

Hr = mRNA stabilizing hairpin

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
    Illegal NotI site found at 58
    Illegal NotI site found at 78
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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