Part:BBa_J133575

Protein Linker SSGSSG Library

Variant library of 6-amino-acid fusion protein linker sequences, encoded with different codon permutations while maintaining the same SSGSSG protein sequence, and the same codon usage count.

Sequences from this library can be picked from when designing a construct containing multiple fusion proteins (e.g. fusing GFP to protein A, RFP to protein B, YFP to protein C, BFP to for protein D, all in the same plasmid). Select a different linker for each fusion rather than reuse multiple copies of the same linker to reduce the chance of homologous recombination/misprimining event/gibson assembly overlap similarity.

Permutations of the codons are generated while following the rules:<br\> 1. encoding only SSGSSG<br\> 2. using only codons below, where codon frequency in E. coli is represented between [], and its symbolic representation between ()<br\> Serine: AGT(S)[8.7%], AGC(s)[16.1%], TCC($)[8.6%]<br\> Glycine: GGC(G)[29.8%], GGT(g)[24.9%]<br\> 3. all above codons can be only used once except for (s), which is used twice<br\> 4. no two of the same codon can be next to each other, in this case no (s)(s) should occur<br\>

Library of all possible 32 permutations of protein linker sequences:<br\> Part_Number: symbolic_representation, sequence_gapped, sequence_ungapped<br\> BBa_J133550: s$gsSG, AGC TCC GGT AGC AGT GGC, AGCTCCGGTAGCAGTGGC<br\> BBa_J133551: s$gSsG, AGC TCC GGT AGT AGC GGC, AGCTCCGGTAGTAGCGGC<br\> BBa_J133552: s$GsSg, AGC TCC GGC AGC AGT GGT, AGCTCCGGCAGCAGTGGT<br\> BBa_J133553: s$GSsg, AGC TCC GGC AGT AGC GGT, AGCTCCGGCAGTAGCGGT<br\> BBa_J133554: sSg$sG, AGC AGT GGT TCC AGC GGC, AGCAGTGGTTCCAGCGGC<br\> BBa_J133555: sSgs$G, AGC AGT GGT AGC TCC GGC, AGCAGTGGTAGCTCCGGC<br\> BBa_J133556: sSG$sg, AGC AGT GGC TCC AGC GGT, AGCAGTGGCTCCAGCGGT<br\> BBa_J133557: sSGs$g, AGC AGT GGC AGC TCC GGT, AGCAGTGGCAGCTCCGGT<br\> BBa_J133558: $sGsSg, TCC AGC GGC AGC AGT GGT, TCCAGCGGCAGCAGTGGT<br\> BBa_J133559: $sGSsg, TCC AGC GGC AGT AGC GGT, TCCAGCGGCAGTAGCGGT<br\> BBa_J133560: $sgsSG, TCC AGC GGT AGC AGT GGC, TCCAGCGGTAGCAGTGGC<br\> BBa_J133561: $sgSsG, TCC AGC GGT AGT AGC GGC, TCCAGCGGTAGTAGCGGC<br\> BBa_J133574: Ssgs$G, AGT AGC GGT AGC TCC GGC, AGTAGCGGTAGCTCCGGC<br\> BBa_J133575: Ssg$sG, AGT AGC GGT TCC AGC GGC, AGTAGCGGTTCCAGCGGC<br\> BBa_J133576: SsGs$g, AGT AGC GGC AGC TCC GGT, AGTAGCGGCAGCTCCGGT<br\> BBa_J133577: SsG$sg, AGT AGC GGC TCC AGC GGT, AGTAGCGGCTCCAGCGGT<br\>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]