Temporary

Part:BBa_J133002

Designed by: Norman Wang   Group: Presting Lab   (2010-12-17)


GFPFrag+OligoN

aka GFPFrag + OligoN Merger of 2 Parts:

1.) PCRed B0034+E0040 5' fragment from E0240 with mutagenesis primers to remove BsmBI

2.) 326bp Synthetic fragment (OligoN) to include: 

a.) 3' fragment of E0040, removing BsaI and

b.) RNAseE+NheI+NsiI+RNAseE+Hairpin (reverse engineered from pSB1A10)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 829
    Illegal NotI site found at 21
    Illegal NotI site found at 835
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 849


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