Project

Part:BBa_J13204

Designed by: Jeffrey J Tabor   Group: iGEM2005   (2005-09-20)


3OC6HSL + LuxR reporter and cI tuning device

This device uses the S03182 module to produce lambda cI in an IPTG (or lactose) dependent fashion. Lambda cI concentration should be tune-able in certain E.coli strains. The I6042 module is used to read out 3OC6HSL and LuxR concentrations.


Usage and Biology

leaky expression from the cI portion of this device (hosted on pSB4A3) is enough to completely quench leaky activity of I15030

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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