Project

Part:BBa_J13202

Designed by: Jeffrey J Tabor   Group: iGEM2005   (2005-09-20)


3OC6HSL + LuxR reporter and aiia (LVA+) tuning device

This device uses the S01656 module to encode IPTG (or lactose) inducible aiia (LVA+), allowing the tuning of 3OC6HSL degradation activity in lactose tune-able E.coli strains. The presence of the 3OC6HSL and the LuxR protein is read out by the I6042 module.


Usage and Biology

AiiA LVA+ in this system is capable of inhibiting a leaky 3OC6HSL promoter significantly. However, it is not active enough to completely inhibit I15030, the strong 3OC6HSL amplifier -JT 10_26_05

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1129
  • 1000
    COMPATIBLE WITH RFC[1000]


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