Project

Part:BBa_J13030

Designed by: Laura Lavery   Group: iGEM2005   (2005-06-22)


AI-1 amplifier

This circuit is regulated by two proteins, TetR and CI. In the presence of TetR, LasR production will be repressed. In the presence of CI, LasI production will be repressed. Without repressors present the circuit may be leaky enough to trigger the positive feedback LasI/LasR/PAI response.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 855
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1408
    Illegal AgeI site found at 417
    Illegal AgeI site found at 1605
  • 1000
    COMPATIBLE WITH RFC[1000]


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