Reporter

Part:BBa_J13000

Designed by: Laura Lavery   Group: iGEM2005   (2005-05-23)


3OC6HSL+LuxR dependent LacZalpha generator

In the presence of 30C6HSL and LuxR this part will generate LacZ alpha peptide. Requires a lacZ delta m15 strain of e.coli.


Usage and Biology

we're pretty sure that E0033 (lacZ alpha) has an internal deletion making it non functional (JT 07.17.05)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 174
    Illegal XhoI site found at 225
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/pret
Parameters
None