Composite

Part:BBa_J120027

Designed by: Sanjana Gurrala, Xiaoyang Feng, Stephen Cain, Orijit Kar, Sahil Sharma   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-03)

It is a composite part containing CH1 domain of human antibody along with GFP.

This composite part contains the CH1 domain of human antibody (Part:BBa_J120020) along with GFP (Part:BBa_K194001) and a yeast terminator (Part:BBa_B0011). For this device to function properly, a promoter and the heavy chain of the variable region(Part:BBa_J120024) are required. Since the sequence of this variable region is not known, we have not included it as part of this composite part. Furthermore, for the antibody recognition to function properly, this part also requires the sequence of the light chain variable region(Part:BBa_J120031) and kappa 1 immunoglobulin light chain constant region of human antibody (Part:BBa_J120021). Once sequenced and linked together this device will recognise and bind to ADAM17. Once the antibody binds to ADAM17, the cells will fluoresce green.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 941


[edit]
Categories
Parameters
n/aIt is a composite part. which contains constant region of ADAM 17 antibody along with GFP (Green Flu