Coding

Part:BBa_J120025

Designed by: Sanjana Gurrala, Xiaoyang Feng, Stephen Cain, Orijit Kar, Sahil Sharma   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-03)

TACE(Tumor necrosis factor-α-converting enzyme) aka ADAM17 endopepsidase (H. sapiens)

ADAM17, also known as tumor necrosis factor alpha; converting enzyme (TACE) is activated by several tyrosine kinase receptors, and is known to be over expressed in cancerous cells. It is an integral transmembrane that produces TNF-alpha; and participates in ectodomain shedding of other transmembrane proteins. This part has to be linked with promoter in order to be expressed in yeast. For the Bio Brick standard, we replaced Pst I sites in the sequence as c to t at 1356, c to t at 1809, g to a at 2220, and g to a at 2436 without changing the amino acids.

References: 

(The coding sequence only is used from the below link)
http://www.ncbi.nlm.nih.gov/nuccore/73747888


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
    Illegal XhoI site found at 94
    Illegal XhoI site found at 1440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 616
    Illegal SapI.rc site found at 641


[edit]
Categories
Parameters
None