Protein_Domain

Part:BBa_J120020

Designed by: Xiaoyang Feng, Orijit Kar, Stephen Cain, Sanjana Gurrala, Sahil Sharma   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-03)

CH1 domain of human antibody

This part is the CH1 domain of human antibody. The constant FC region is not required for the attachment of other subparts.The antibody can still function in the same manner even if there is no FC region present. The protein sequence taken from the reference is from 1-98. In addition, we have added a start codon at the beginning and STOP codon has been removed such that our composite part is functional.

References:

(CH1 protein region is from 1 to 98) 

http://www.uniprot.org/uniprot/P01857

(CH1 gene sequence from 1 to 294bp based on the protein) 

http://www.ncbi.nlm.nih.gov/nucleotide/12054071?report=genbank&log$=nucltop&blast_rank=18&RID=S8U5XUKA01N

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 108
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHeavy Chain Constant region of ADAM17 monoclonal antibody (M01), clone 1F6