Composite

Part:BBa_J120014

Designed by: Brett Fuller, Austin Jones, Jeremy Gerbig, Sindhu Adhikari, Coby Turner   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-03)


Binding sites for Pax3 and Sox10 with GFP selectable marker and double terminator

Contains two short binding sites (BBa_J120012 and BBa_J120013) followed by a GFP selectable marker (BBa_K259006). When these binding sites have their respective transcription factors bound to them, they will induce the production of GFP, which is a visually detectable marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 695


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Categories
Parameters
None