Coding

Part:BBa_J120000

Designed by: Corinthia Black, Sheree Harper, Srikanth Sistla, Anna Wieman   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-10)

Coding sequence for PARP1 (poly [ADP-ribose] polymerase 1) H. sapiens

Coding sequence for PARP1 (poly[ADP-ribose] polymerase 1) which is instrumental in detecting damaged DNA, directly binding to it and catalyzing its repair. During apoptosis, PARP1 is typically cleaved, inhibiting its function (Oliver et al., 1999). The sequence was originally obtained from NCBI (accession # NM_001618.3). This sequence was modified to remove internal restriction sites by changing the third base of the codons at these sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1599
    Illegal BamHI site found at 2361
    Illegal BamHI site found at 2612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2700
    Illegal BsaI.rc site found at 2738


[edit]
Categories
Parameters
None