Regulatory

Part:BBa_J119405

Designed by: Lindsay Doolan and the fall 2016 Missouri Western Genetics class   Group: Eckdahl Lab   (2017-03-24)


(pSB1A2-BR) Deletion of the original -35 sequence

The Pupp promoter responds to UTP (see BBa_J100305). This mutant has a deletion of the original -35 element (TGCCGC) to test the effects on transcription with a different -35 sequence (AAGAAT).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None