Device

Part:BBa_J119402

Designed by: Anthony J. Eckdahl   Group: Eckdahl Lab   (2016-08-01)


Double NiCo Riboswitch in tClone Red

A riboswitch found to be responsive to transitions metals (Ni++, Co++, Mn++) from Furakawa et al. (Molecular Cell 57: 1088-1098, 2015) was cloned as a tandem repeat into tClone Red using BsaI Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 938
    Illegal AgeI site found at 1050
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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