Plasmid

Part:BBa_J119399

Designed by: Andrew Hunn   Group: Eckdahl Lab   (2016-07-18)


RS10 in J100270 Selection Phage

Riboswith 10, from Wachsmuth et. al., inserted into J100270 selection phage between the Kanamycin resistance gene and the T7 RNAP gene. In the presence of Theophylline, this part should produce the T7 RNAP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5551
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5551
    Illegal NgoMIV site found at 8513
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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