Device

Part:BBa_J119396

Designed by: Athena J. Davis   Group: Eckdahl_Lab   (2016-06-24)


SacB + PDB riboswitch

The PDB riboswitch consists of a theophylline aptamer sequence from the Protein Databank. This aptamer sequence was inserted into the Wachsmuth et al. RS3 riboswitch sequence using Golden Gate Assembly. The SacB plasmid backbone was obtained from part J119042, and Golden Gate Assembly was used to ligate the riboswitch into the SacB plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1417
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1391
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None