Device

Part:BBa_J119395

Designed by: Todd Eckdahl   Group: Eckdahl_Lab   (2016-06-23)


tClone Red containing the RS3 Riboswitch

The RS3 riboswitch from Wachsmuth et. al. (2013) cloned into tClone Red using Golden Gate Assembly. The RS3 riboswitch is a theophylline riboswitch that is on half of the time when theophylline is not present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 765
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None