Regulatory

Part:BBa_J119382

Designed by: MWSU Genetics Spring 2015 Thursday 8:00 lab   Group: Eckdahl Lab   (2015-04-06)


Ptac mutant A4ins

This part was designed and clone by students in the Missouri Western State University Genetics class of Spring 2015. It was cloned into the pClone Red vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation inserts AAAA at position -14. The mutation had a large effect on the function of the promoter (see Experience page).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None