Regulatory

Part:BBa_J119372

Designed by: MWSU Genetics Fall 2014 Thursday 8:00 lab   Group: Eckdahl Lab   (2014-11-07)


Ptac mutant -34 TT Deletion and -28 GGC Insertion

This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2014. It was cloned into the pClone Red vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation deletes TT at position -34 and inserts GGC at position -28. The mutation had a large effect on the function of the promoter (see Experience page).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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