Plasmid

Part:BBa_J119366

Designed by: Todd Eckdahl   Group: Eckdahl_Lab   (2014-08-06)


pSB1A2-BR

This plasmid is pSB1A2 with its BsaI site removed (BR = BsaI Removed), so it can be used in BsaI Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2058
    Illegal XbaI site found at 2073
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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