Measurement

Part:BBa_J119364

Designed by: Kelly Cochran, Mike Quaney   Group: Eckdahl_Lab   (2014-07-22)


Ammeline Fitness Module with Riboswitch N2 + TetA (T138C)

The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2 with mutation T138C) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 506
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1034
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None