Device

Part:BBa_J119347

Designed by: Brandon Grieshaber   Group: Eckdahl Lab   (2014-04-17)

eCDM8 expression + Theophylline riboswitch tetracycline resistance protein + GFP expression

Low strength promoter (J119030) and a low strength C dog RBS (J119025) controlling expression of the eCDM8 gene (J100100). The clone also contains theophylline riboswitch D (Part J100065) and tetracycline resistance protein (Part J119140) and RFP expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
    Illegal BamHI site found at 3737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3763
    Illegal NgoMIV site found at 4131
    Illegal NgoMIV site found at 4291
    Illegal AgeI site found at 199
    Illegal AgeI site found at 860
    Illegal AgeI site found at 3385
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5377


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