Device

Part:BBa_J119340

Designed by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige   Group: Eckdahl Lab   (2014-02-07)


Ammeline Fitness Module with Riboswitch M6 double prime + ThyA

The P5 promoter drives a riboswitch (M6 double prime from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a ThyA (thymidylate synthase) fitness gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 789
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None