Device

Part:BBa_J119331

Designed by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige   Group: Eckdahl Lab   (2014-02-03)


Ammeline Biosensor with Riboswitch AddA + RFP

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (AddA from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 740
    Illegal AgeI site found at 852
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None