Measurement

Part:BBa_J119328

Designed by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige   Group: Eckdahl Lab   (2013-12-26)


Ammeline Fitness Module with Riboswitch + TetA

The P5 promoter drives a riboswitch (M6C) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 504
    Illegal NgoMIV site found at 872
    Illegal NgoMIV site found at 1032
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None