Regulatory

Part:BBa_J119327

Designed by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige   Group: Eckdahl Lab   (2013-12-19)


P5 promoter + Ammeline Riboswitch M6C

The P5 promoter drives a riboswitch (M6C) that allows tranlsation in the presence of ammeline (made by demethylation of melamine). The clone also has a BsaI GGA site for insertion of a fitness or reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 200
    Illegal BsaI.rc site found at 190


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Categories
Parameters
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