DNA

Part:BBa_J119316

Designed by: Todd Eckdahl   Group: Eckdahl_Lab   (2013-10-16)


Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)

Scaffold 2.0 with the RBS in the junction B.The vector is prepared by inverted PCR (iPCR) using primers with BsaI sites that produce 5' overhanging sticky ends for the junctions B and Junction C. The RBS has the same sticky ends.J-GGA is then performed with BsaI and T4 DNA Ligase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 214
    Illegal NotI site found at 6
    Illegal NotI site found at 221
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 214
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None