DNA

Part:BBa_J119315

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2013-10-05)


Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)

Scaffold 2.0 with the promoter in the junction A.The vector is prepared by inverted PCR (iPCR) using primers with BsaI sites that produce 5' overhanging sticky ends for the junctions A and Junction B. The promoter has the same sticky ends.J-GGA is then performed with BsaI and T4 DNA Ligase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 139
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 139
    Illegal NotI site found at 6
    Illegal NotI site found at 146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 139
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None