DNA

Part:BBa_J119314

Designed by: Todd Eckdahl   Group: Eckdahl_Lab   (2013-10-05)


Scaffold 2.0 for J-GGA

Scaffold 2.0 allows the use of BsaI Junction Golden Gate Assembly (J-GGA) to build a gene expression cassette. The vector is prepared by inverted PCR (iPCR) using primers with BsaI sites that produce 5' overhanging sticky ends for the junctions involved. The insert either has the same sticky ends or carries BsaI sites that produce them. J-GGA is then performed with BsaI and T4 DNA Ligase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 107
    Illegal NotI site found at 6
    Illegal NotI site found at 114
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None