Plasmid

Part:BBa_J119312

Designed by: Brandon Grieshaber and Todd Eckdahl   Group: Eckdahl Lab   (2013-07-29)


pSB1A2-BR-HS-T-RNA-I

This vector is pSB1A2 with its BsaI site removed (BR = BsaI Removed), so it can be used in BsaI Golden Gate Assembly. It also has been verified to have an T at position -1 of the RNA I gene in the pMB1 origin of replication. This site has been identified as a hotspot for mutation of the pMB1 origin (Lin-Chao et al. 1992).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2058
    Illegal XbaI site found at 2073
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None