Plasmid

Part:BBa_J119311

Designed by: Brandon Grieshaber and Todd Eckdahl   Group: Eckdahl Lab   (2013-07-11)


pSB1A2-BR-P5-RNA-I

This vector is pSB1A2 with its BsaI site removed (BR = BsaI Removed), so it can be used in BsaI Golden Gate Assembly. It also has a P5 promoter (BBa_J119031) in place of the natural promoter for RNA I in the pMB1 origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2045
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2045
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2045
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2045
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2045
    Illegal XbaI site found at 2060
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None