Reporter

Part:BBa_J119306

Designed by: Sachii Polpitiya Arachchige   Group: Eckdahl_Lab   (2013-07-02)


RFP expression cassette for J-GGA

The scaffold for Junction Golden Gate Assembly (J-GGA) is populated with a promoter related to P5 (J110031), a C dog RBS element related to BD2 (J119025), and a mRFP codon optimized for E. coli. J-GGA can be used to vary any of the elements. Inverted PCR from two junctions is followed by BsaI/Ligase assembly of elements carrying the appropriate BsaI sites or sticky ends.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 720
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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