Reporter
Part:BBa_J119306
Designed by: Sachii Polpitiya Arachchige Group: Eckdahl_Lab (2013-07-02)
RFP expression cassette for J-GGA
The scaffold for Junction Golden Gate Assembly (J-GGA) is populated with a promoter related to P5 (J110031), a C dog RBS element related to BD2 (J119025), and a mRFP codon optimized for E. coli. J-GGA can be used to vary any of the elements. Inverted PCR from two junctions is followed by BsaI/Ligase assembly of elements carrying the appropriate BsaI sites or sticky ends.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 720
Illegal AgeI site found at 832 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |